ACDC DATA
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Doulix Biomodules Collection
We provide the first and only collection of certified standard biological parts, called BIOMODULEs. Certified public BIOMODULEs are sequence-verified and tested in vivo for function before release.
Our lab tests are carried out under the most stringent ISO-9001 quality controls.
Browse our broad collection of promoters, RBSs, terminators and reporting genes. If you can’t find what you’re looking for, log-in and design your own BIOMODULES. -
Doulix Constructs Collection
We provide the first and only collection of certified full length constructs. Certified public CONSTRUCTS are sequence-verified before release.
Our lab tests are carried out under the most stringent ISO-9001 quality controls.
If you can’t find what you’re looking for, log-in and design your own CONSTRUCTS. -
ACDC Chemical Compiler
This is the export of the "final" branch of the ACDC Chemical Compiler. For more information, see the included documentation and the corresponding publication https://doi.org/10.1162/isal_a_00648.
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Fortran program for simulating the Kauffman model with multiple containers and spatially separated ligation and cleavage processes
The file copolymer.f contains a Fortran77 program (using Fortran8.x extensions) simulating the Kauffman model in a large number of containers, connected by diffusion processes, with spatially separated ligation and cleavage processes. For further information, see the file readme.txt and the comments in copolymer.f
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Artificial chemistry programs
Compressed file containing three executables anf an input file for Ubuntu Linux, simulating a toy example for artificial chemistry, of a two-step reaction system with an undesired side product, as described in the artificial chemistry paper by Johannes Josef Schneider et al. in the Wivace 2022 conference proceedings
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Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis - software
Scripts developed for the analysis of vesicles fusion experiments by high-throughput image analysis for "Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis".
- Quick User Guide for GUV fusion Analysis.pdf is a short manual detailing the main steps to complete the analisys of imaging data by our system
- GUVfusion_Analysis.ijm is the ImageJ macro used to segment GUVs
- GUVfusion_AnalysisTemplate.R is the R template script for data elaboration and visualization. It makes use of the other two R scripts included
- GUVfusion_dataElabFunctions.R contains data elaboration functions to import raw csv and tiff data in R
-GUVfusion_plotFunctions.R contains data visualization functions.
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Instructions for the construction of a Low cost Lego 3D bioprinter
Parts list and an instructional video for the recreation of a Lego 3D bioprinter.
When referencing please use:
Moukachar, A. et al. Development and Evaluation of a Low-Cost LEGO 3D Bioprinter: From Building-Blocks to Building Blocks of Life. Advanced Materials Technologies 8, 2100868 (2023).
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Video data describing bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells
Video data of loop-mediated isothermal nucleic acid amplification (LAMP) and bioluminescent assay in real time (BART) in microfluidicaly produced droplets. The videos are the data used to generate still images for figures in the source publication "Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells".
Details of the videos acquisition parameters are as follows:
- Video 1: LAMP-BART droplets in tubing - no DNA template control (Figure 2a) (assay duration 600s, frames 1 per 10s, 60 frames, final
- Video 2 frames per second) Video 2: LAMP-BART droplets in tubing - positive DNA template control (Figure 2b) (assay duration 1800s, frames 1 per 10s, 180 frames, final video 10 frames per second)
- Video 3: LAMP-BART parked droplets (Figure 3) (assay duration 600s, frames 1 per 10s, 60 frames, final video 3.3 frames per second)
- Video 4: LAMP-BART DNA gradient in tubing (Figure 4) (assay duration 1800s, frames 1 per 10s, 180 frames, final video 10 frames per second)
- Video 5: LAMP-BART eDIBs (Figure 5) (assay duration 1570s, frames 1 per 10s, 157 frames, final video 5.2 frames per second)
- Video 6: One to four core LAMP-BART eDIBs (Figure 6) (assay duration 1200s, frames 1 per 10s, 120 frames, final video 5 frames per second)
When referencing please use:
Hardinge, P., Baxani, D. K., McCloy, T., Murray, J. A. H. & Castell, O. K. Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells. Sci Rep 10, 21886 (2020).